CsCl gradient solutions

CsCl

0.02 M Tris-Cl (pH 7.4)

For a 1.28 g/mL solution, dissolve 30 g of solid CsCl in 70 mL of 0.02 M Tris-Cl (pH 7.4).

For a 1.50 g/mL solution, dissolve 40 g of solid CsCl in 54 mL of 0.02 M Tris-Cl (pH 7.4).

Weigh 1-mL aliquots of each solution on an analytical balance to confirm densities. Filter-sterilize through a 0.22-μm filter.