Recipe

SDS solubilization buffer (1X)

Reagent Quantity
(for 500 mL) 		Final concentration
EDTA (500 mM, pH 8.0) 1 mL 1 mM
SDS (sodium dodecyl sulfate; 10%) 25 mL 0.5%
Tris-Cl (1 M, pH 7.5) 10 mL 20 mM
H2O, RNase-free 464 mL --
Store for up to 1 yr at room temperature.
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  1. doi:10.1101/pdb.rec12227 Cold Spring Harb Protoc 2010: pdb.rec12227-
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