Recipe

Acrylamide stock for RNA gels (40%)

Reagent Quantity (for 500 mL) Final concentration
Acrylamide (electrophoresis grade) 190 g 38% (w/v)
Bisacrylamide (electrophoresis grade) 10 g 2% (w/v)
H2O, RNase-free to 500 mL --
Filter with a 0.45-μm filter and store at 4°C in a dark bottle.
Final solution is 19:1 acrylamide:bisacrylamide.
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  1. doi:10.1101/pdb.rec12230 Cold Spring Harb Protoc 2010: pdb.rec12230-
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