RNA Gel-Loading Buffer (1.5×)
| Reagent | Quantity (for 10 mL of 1.5×) | Final concentration |
|---|---|---|
| Formamide, ultrapure | 9.5 mL | 95% |
| Bromophenol blue (2.5%, w/v) | 100 µL | 0.025% |
| Xylene cyanol FF (2.5%, w/v) | 100 µL | 0.025% |
| EDTA (0.25 m, pH 8.0) | 200 µL | 5 mm |
| Use 5 µL for a 2.5-μL sample. Purchase a distilled, deionized preparation of formamide and the above loading dyes. Store in small (1-mL) aliquots for up to 1 yr at −20°C. This solution is available commercially (Ambion) and is recommended over homemade. | ||
- © 2010 Cold Spring Harbor Laboratory Press
