Recipe

Disruption buffer

5 μg/mL chymostatin

2 mM DTT (dithiothreitol)

10 mM EDTA

40 mM HEPES (pH 7.2)

10 μg/mL leupeptin

5 μg/mL pepstatin

Prepare fresh and keep at 4°C.

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  1. doi:10.1101/pdb.rec12238 Cold Spring Harb Protoc 2010: pdb.rec12238-
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