Recipe

FPLC gel filtration buffer

1 mM DTT (dithiothreitol)

50 mM HEPES (pH 7.2)

250 mM KCl

0.2 mg/mL rcm-BSA (bovine serum albumin, reduced, carboxymethylated)

Prepare fresh and keep at 4°C.

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  1. doi:10.1101/pdb.rec12239 Cold Spring Harb Protoc 2010: pdb.rec12239-
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