Recipe

Buffer B (FPLC Mono Q)

1 mM DTT (dithiothreitol)

1 M NaCl

50 mM Tris-Cl (pH 7.2)

Prepare fresh and keep at 4°C.

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  1. doi:10.1101/pdb.rec12242 Cold Spring Harb Protoc 2010: pdb.rec12242-
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