Table

Table 1. Commonly used SNV-based vectors and packaging cell lines

Vector Description Reference
SNV-based avian retroviral vectors
pJD214 SNV-based splicing vector containing ~1 kb of virus sequence Dougherty and Temin (1987)
pMH105 Bicistronic SNV retroviral vector containing v-rel-IRES-neo White and Gilmore (1996)
pJD220SVHy SNV-based vector containing an internal SV40 promoter expressing the hygromycin phosphotransferase B gene (hygro) Dougherty and Temin (1987)
pIM29 Expresses SNV wild-type SU region of envelope Martinez and Dornburg (1995a,b)
pRD134 SNV envelope plasmid expressing wild-type proteins Martinez and Dornburg (1995a,b)
pRD136 SNV packaging construct expressing wild-type gag-pol Martinez and Dornburg (1995a,b)
pZP32 SNV packaging construct with mutation in matrix region Parveen et al. (2000, 2003)
pZP36 SNV-based retroviral vector used for generation of viral particles expressing LacZ Parveen et al. (2003)
pEM12 and 13 Bicistronic vectors expressing anti-HIV-1 genes Marusich et al. (2005)
Packaging cell lines for SNV-based vectors
D17 Canine osteosarcoma cell line, permissive to infection by SNV, REV-A, and murine leukemia virusa Riggs et al. (1974)
C3A2 A derivative of D17 cell line expressing REV-A gag-pol and env Watanabe and Temin (1983)
DSH134G SNV-based packaging cell line Martinez and Dornburg (1995a,b)
DSH-cxl SNV-based packaging cell line with β-gal as marker
aREV-A and SNV are >90% homologous. REV-A proteins have also been reported to package SNV-derived vectors with high efficiency (Yin and Hu 1997).
| Table of Contents

This Article

  1. doi:10.1101/pdb.tab195435 Cold Spring Harb Protoc 2010: pdb.tab195435-

Article Category

  1. Table

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Alert me when Updates/Comments are published

Share