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Cover Illustration: 3D image projection of a confocal stack of a fluorescently labeled ventral nerve cord of a Drosophila third instar larva. The synaptic neuropil is labeled by α-Bruchpilot antibody (blue), cell bodies of gabaergic neurons are highlighted by α-GABA antibody (red), and single neurons are labeled by GFP expression (green) using a Flp-out strategy (Genotype: gad-Gal4/ UAS>CD2>CD8GFP; UAS FLP). (Image courtesy of Tobias Stork and Marc R. Freeman.) For more information on this method, see Immunofluorescent Staining of Drosophila Larval Brain Tissue (doi:10.1101/pdb.prot5460).