Recipe

Peptide dilution buffer

0.2 mg/mL bovine serum albumin (BSA)

20 mM HEPES-KOH (pH 7.9)

20% glycerol (v/v)

0.1 mM EDTA

0.25 M KCl

0.05% Nonidet P-40 (NP-40)

1 mM PMSF (phenylmethylsulfonyl fluoride)

Add PMSF just before use.

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  1. doi:10.1101/pdb.rec11272 Cold Spring Harb Protoc 2010: pdb.rec11272-
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