| EDTA (0.5 M)
|
10 mL |
10 mM |
| SDS (10%)
|
250 mL |
5% |
| Tris-Cl (1 M, pH 7.5)
|
100 mL |
200 mM |
| H2O, RNase-free
|
140 mL |
|
|
| All reagents used to make this buffer should be prepared with RNase-free H2O.
|
| Store for up to 1 yr at room temperature. Dilute 1:10 as needed to make 1X. |