Recipe

Homogenization buffer A

2 mM EDTA

150 mM NaCl

50 mM Tris-Cl (pH 7.5)

0.5 mM DTT (dithiothreitol)

Prepare a 0.5 M stock solution of DTT in cold H2O; store frozen. Just before use, add the DTT to the chilled buffer at the indicated concentration.

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  1. doi:10.1101/pdb.rec12251 Cold Spring Harb Protoc 2010: pdb.rec12251-
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