Recipe

Homogenization buffer A

2 mM EDTA

150 mM NaCl

50 mM Tris-Cl (pH 7.5)

0.5 mM DTT (dithiothreitol)

Prepare a 0.5 M stock solution of DTT in cold H2O; store frozen. Just before use, add the DTT to the chilled buffer at the indicated concentration.

No Related Web Pages
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec12251 Cold Spring Harb Protoc 2010: pdb.rec12251-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Related Content

  1. Related Web Pages

Share

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue