Recipe

Fix solution

Reagent Quantity (for 10 mL) Final concentration
Formaldehyde (37%, v/v) 1.1 mL 4% (v/v)
PIPES (1 M, pH 6.9) 1.0 mL 0.1 M
Triton X-100 (10%, v/v) 0.3 mL 0.3% (v/v)
EGTA (0.1 M, pH 8.0) 0.2 mL 20 mM
MgSO4 (1 M) 10 μL 1 mM
H2O 7.4 mL
Prepare fresh prior to use.
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  1. doi:10.1101/pdb.rec12270 Cold Spring Harb Protoc 2010: pdb.rec12270-

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