Recipe

Carbonate buffer (2X)

Reagent Quantity (for 100 mL) Final concentration
Na2CO3 0.636 g 60 mM
NaHCO3 0.336 g 40 mM
Prepare in H2O. Adjust the pH to 10.2. Store at room temperature.
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  1. doi:10.1101/pdb.rec12273 Cold Spring Harb Protoc 2010: pdb.rec12273-
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