| Animal tissues |
AEBSF (0.2 mM) or DCI (0.1 mM) or PMSF (0.2 mM) |
Serine |
AEBSF: 20 mM in methanol |
|
|
|
DCI: 10 mM in DMSO |
|
|
|
PMSF: 200 mM in ethanol or isopropanol |
|
Benzamidine (1 mM) |
Serine |
100 mM |
|
Leupeptin (10 μg/mL) |
Serine/cysteine |
1 mg/mL |
|
Pepstatin (10 μg/mL) |
Aspartic |
5 mg/mL in methanol |
|
Aprotinin (1 μg/mL) |
Serine |
0.1 mg/mL |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
|
|
|
|
| Plant tissues |
AEBSF (0.2 mM) or DCI (0.1 mM) or PMSF (0.2 mM) |
Serine |
AEBSF: 20 mM in methanol |
|
|
|
DCI: 10 mM in DMSO |
|
|
|
PMSF: 200 mM in ethanol or isopropanol |
|
Chymostatin (20 μg/mL) |
Serine/cysteine |
1 mg/mL in DMSO |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
|
|
|
|
| Yeasts and fungi |
AEBSF (0.2 mM) or DCI (0.1 mM) or PMSF (0.2 mM) |
Serine |
AEBSF: 20 mM in methanol |
|
|
|
DCI: 10 mM in DMSO |
|
|
|
PMSF: 200 mM in ethanol or isopropanol |
|
Pepstatin (15 μg/mL) |
Aspartic |
5 mg/mL in methanol |
|
1,10-phenanthroline (5 mM) |
Metallo |
1 M in ethanol |
|
|
|
|
| Bacteria |
AEBSF (0.2 mM) or DCI (0.1 mM) or PMSF (0.2 mM) |
Serine |
AEBSF: 20 mM in methanol |
|
|
|
DCI: 10 mM in DMSO |
|
|
|
PMSF: 200 mM in ethanol or isopropanol |
|
EDTA or EGTAc (1 mM)
|
Metallo |
100 mM |
|
| Adapted from North (1989). |
| Abbreviations: AEBSF: 4-(2-aminoethyl)-benzenesulfonyl fluoride; DCI: 3,4-dichloroisocoumarin; DMSO: dimethyl sulfoxide; EDTA:
ethylenediamine tetraacetic acid; EGTA: ethylene glycol bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid; PMSF: phenylmethylsulfonyl fluoride.
|
| Mr values of inhibitors: 1,10-phenanthroline: 198; AEBSF: 240; aprotinin: 6500; benzamidine hydrochloride: 157; chymostatin:
605; DCI: 215; EDTA (disodium salt, dihydrate): 372; leupeptin: 427; pepstatin: 686; PMSF: 174.
|
| aFor a review of proteolytic enzymes, see Neurath (1989) and Perlmann and Lorand (1970).
|
| bAqueous solution unless otherwise indicated.
|
| cAn efficient chelator of divalent metal cations other than Mg2+ (for which it has a 103-fold lower affinity) (Gegenheimer 1990).
|
