Table

Table 1. RNA isolation kits

Kit name Lysis buffer Selectivity Binding chemistry
RiboPure (Ambion) TRIzol Total RNA (not selective) Silica gel/glass fiber in high salt and ethanol
TRIzol-Plus (Invitrogen) TRIzol Total RNA (not selective) Silica gel/glass fiber in high salt and ethanol
ChargeSwitch (Invitrogen) Urea-LiCl Total RNA (not selective) Organic pH-dependent anion exchange matrix coated on paramagnetic beads
RNaqueous (Ambion) GITC Total RNA (not selective) Silica gel/glass fiber in high salt and ethanol
RNeasy (QIAGEN) GITC Large RNAs (>200 nt) Silica gel/glass fiber in high salt and ethanol
mirVana (Ambion) GITC Small RNAs Silica gel/glass fiber in high salt and ethanol
miRNeasy (QIAGEN) GITC Inclusive of small RNAs, not selective (total RNA) (total RNA) Silica gel/glass fiber in high salt and ethanol
Purelink miRNA (Invitrogen) GITC Small RNAs Silica gel/glass fiber in high salt and ethanol
Oligotex poly(A)+ (QIAGEN) GITC, urea-SDS, or purified total RNA Poly(A)+ RNAs dC10dT30 covalently linked to polystyrene-latex beads
DynaBeads mRNA DIRECT (Invitrogen) 0.5 M LiCl, lithium DS Poly(A)+ RNAs dT25 attached to paramagnetic beads
RiboMinus (Invitrogen) Purified total RNA in solution Non-rRNAs 5′-Biotinylated LNA oligonucleotides bound to streptavidin-linked paramagnetic beads
(GITC) Guanidinium isothiocyanate, (lithium DS) lithium dodecyl sulfate, (LNA) locked nucleic acid, (nt) nucleotide(s), (TRIzol) a monophasic mixture of phenol and guanidinium isothiocyanate.
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  1. doi:10.1101/pdb.tab1ip79 Cold Spring Harb Protoc 2010: pdb.tab1ip79-

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