| RiboPure (Ambion) |
TRIzol |
Total RNA (not selective) |
Silica gel/glass fiber in high salt and ethanol |
| TRIzol-Plus (Invitrogen) |
TRIzol |
Total RNA (not selective) |
Silica gel/glass fiber in high salt and ethanol |
| ChargeSwitch (Invitrogen) |
Urea-LiCl |
Total RNA (not selective) |
Organic pH-dependent anion exchange matrix coated on paramagnetic beads |
| RNaqueous (Ambion) |
GITC |
Total RNA (not selective) |
Silica gel/glass fiber in high salt and ethanol |
| RNeasy (QIAGEN) |
GITC |
Large RNAs (>200 nt) |
Silica gel/glass fiber in high salt and ethanol |
| mirVana (Ambion)
|
GITC |
Small RNAs |
Silica gel/glass fiber in high salt and ethanol |
| miRNeasy (QIAGEN) |
GITC |
Inclusive of small RNAs, not selective (total RNA) (total RNA) |
Silica gel/glass fiber in high salt and ethanol |
| Purelink miRNA (Invitrogen) |
GITC |
Small RNAs |
Silica gel/glass fiber in high salt and ethanol |
| Oligotex poly(A)+ (QIAGEN)
|
GITC, urea-SDS, or purified total RNA |
Poly(A)+ RNAs
|
dC10dT30 covalently linked to polystyrene-latex beads |
| DynaBeads mRNA DIRECT (Invitrogen) |
0.5 M LiCl, lithium DS |
Poly(A)+ RNAs
|
dT25 attached to paramagnetic beads |
| RiboMinus (Invitrogen) |
Purified total RNA in solution |
Non-rRNAs |
5′-Biotinylated LNA oligonucleotides bound to streptavidin-linked paramagnetic beads |
|
| (GITC) Guanidinium isothiocyanate, (lithium DS) lithium dodecyl sulfate, (LNA) locked nucleic acid, (nt) nucleotide(s), (TRIzol)
a monophasic mixture of phenol and guanidinium isothiocyanate.
|