Choline Acetyltransferase Staining in Brain Sections
Adapted from Discovering Neurons: The Experimental Basis of Neuroscience (ed. Paul et al.). CSHL Press, Cold Spring Harbor, NY, USA, 1997.INTRODUCTION
This protocol describes the use of immunocytochemical methods to stain for choline acetyltransferase (ChAT). The avidin-biotin horseradish peroxidase complex (ABC) triple-labeling method used here takes advantage of the extraordinary affinity between avidin and biotin. Horseradish peroxidase attached to biotin in the complex is developed using diaminobenzidine (DAB).
