Recipe

Buffer D+K

20 mm HEPES-KOH (pH 7.9)

20% glycerol (v/v)

0.1 mm EDTA

0.1 M KCl

1 mm DTT (dithiothreitol)

1 mm PMSF (phenylmethylsulfonyl fluoride)

Add the DTT and PMSF just before use.

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  1. doi:10.1101/pdb.rec11271 Cold Spring Harb Protoc 2010: pdb.rec11271-
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