Recipe

Bead washing buffer

0.625X Buffer D+K

7.5 mM MgCl2

0.05% Nonidet P-40

Immediately before use, add

1 mM DTT (dithiothreitol)

1 mM PMSF (phenylmethylsulfonyl fluoride)

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  1. doi:10.1101/pdb.rec12281 Cold Spring Harb Protoc 2010: pdb.rec12281-
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