Recipe

Ca2+-free saline

Reagent Quantity (for 1 L) Final concentration
NaCl 7.48 g 128 mM
KCl 0.149 g 2 mM
MgCl2 (1 M) 4 mL 4 mM
Sucrose 12.16 g 35.5 mM
HEPES 1.19 g 5 mM
Dissolve the reagents in ~950 mL of H2O. Add 0.8 g of EGTA (to give a final concentration of 1 mM). Bring the solution to pH 7.2 with NaOH. Note that the EGTA will take a few minutes to dissolve and that it changes the pH as it dissolves. Adjust the final volume to 1 L with H2O.
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec12284 Cold Spring Harb Protoc 2010: pdb.rec12284-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue