α-Chloralose

Recipe

Reagent		Amount to add
	Initial bolus (20 mg/mL)		Maintenance solution (5 mg/mL)
α-Chloralose	0.2 g		0.1 g
Phosphate-buffered saline (PBS)	10 mL		20 mL

1. Combine α-chloralose and PBS in a 50-mL Erlenmeyer flask. Add a stir bar then close with a stopper.
2. Add 40 mL of water to a 100-mL beaker. Place the 50-mL flask in the water with a thermometer.
3. Heat the solution to 80°C, stirring vigorously until the solute is fully dissolved and there is no clouding.
4. Cool to room temperature. Filter the 20-mg/mL solution through a 0.22-μm Millipore filter before administering it.