Dish setup for cultured kidneys. (A,B) Top and (C) side views of complete center-well dishes. Metal mesh is cut to fit inside of the center well, on top of culture medium. Filters are placed over the hole in the center of the mesh with medium contacting, but not covering, the filter (A, dashed line). (D) Filter after 48 h of culture. Up to five kidneys (black arrow) can be comfortably spaced on the filter.