Recipe

Bath solution

5 mM alanine

4 mM CaCl2

5 mM KCl

4 mM MgCl2

120 mM NaCl

25 mM proline

10 mM TES

Adjust the pH to 7.15 and the osmolarity to 82 mOsm.

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  1. doi:10.1101/pdb.rec12387 Cold Spring Harb Protoc 2011: pdb.rec12387-
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