Recipe

Trituration solution

10% fetal calf serum

5 mM KCl

4 mM MgCl2

120 mM NaCl

40 mM sucrose

10 mM TES

Adjust the pH to 7.15.

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  1. doi:10.1101/pdb.rec12389 Cold Spring Harb Protoc 2011: pdb.rec12389-
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