Immunostaining of Drosophila Testes
Adapted from Drosophila Protocols (ed. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.Abstract
Fixed Drosophila testis preparations can be immunostained with a variety of antibodies. This article describes a general procedure for immunostaining. The concentration of the primary antibody will vary with both the type of antibody and the type of incubation and should be determined empirically each time.
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