Recipe

Standard external saline for mouse

125 mm NaCl

4.5 mm KCl

26 mm NaHCO3

1.25 mm NaH2PO4

2 mm CaCl2

1 mm MgCl2

20 mm glucose

The pH should be 7.4 when the solution is bubbled with 95% O2 and 5% CO2.

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  1. doi:10.1101/pdb.rec066456 Cold Spring Harb Protoc 2011: pdb.rec066456- © 2011 Cold Spring Harbor Laboratory Press

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