Recipe

Artificial seawater-HEPES (ASWH)

Reagent Amount to add for 1 L Final concentration
NaCl 24.55 g 420 mm
KCl 0.67 g 9 mm
CaCl2 • 2H2O 1.47 g 10 mm
MgCl2 • 6H2O 4.48 g 24.5 mm
MgSO4 • 7H2O 6.29 g 25.5 mm
NaHCO3 0.18 g 2.15 mm
HEPES (1 m, pH 8.0) 5 mL 5 mm

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  1. doi:10.1101/pdb.rec066720 Cold Spring Harb Protoc 2011: pdb.rec066720- © 2011 Cold Spring Harbor Laboratory Press
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