Recipe

PBS (A)

Reagent Amount to add for 1 L Final concentration
NaCl     8.0 g   137.0 mm
KCl     0.2 g    2.7 mm
KH2PO4     0.2 g    1.5 mm
NaH2PO4    1.14 g    8.0 mm
Adjust pH to 7.2 with HCl (∼4–6 droplets of 6 m HCl).
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  1. doi:10.1101/pdb.rec066753 Cold Spring Harb Protoc 2011: pdb.rec066753- © 2011 Cold Spring Harbor Laboratory Press
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