Topic Introduction

First Steps for Fluorescence Correlation Spectroscopy of Living Cells

  1. Shintaro Mikuni
Adapted from Live Cell Imaging, 2nd edition (ed. Goldman et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2010.

Abstract

Fluorescence correlation spectroscopy (FCS) has become a powerful and sensitive tool in biochemistry and biophysics. It directly obtains physical parameters such as the average number of fluorescent molecules and their diffusion time in a tiny detection area. It also provides other useful information such as the brightness of molecules. Ultimately, it can give precise information about molecular interactions in the aqueous condition. This article outlines the basic parameters and properties of FCS.

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  1. doi:10.1101/pdb.top065920 Cold Spring Harb Protoc 2011: pdb.top065920- © 2011 Cold Spring Harbor Laboratory Press
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