aCSF (1×)
100 mL aCSF stock solution A (10×)
100 mL aCSF stock solution B (10×)
4.5 g glucose
600 mL H2O
Adjust the final volume to 1 L with H2O. Mix well and bubble with 95% O2/5% CO2. Check an aliquot of the 1× aCSF after making each new batch of stock solutions to confirm that the aCSF has a pH of 7.4 and an osmolarity of 310 mOsm when bubbled at room temperature with 95% O2/5% CO2.
- © 2011 Cold Spring Harbor Laboratory Press
