Movement artifacts in 3D image acquisition. The top row shows three adjacent planes from a stack of 17 focal planes of T. gondii expressing a GFP fusion protein that highlights the conoid and other structures. Small movements, difficult to appreciate in single images but obvious when the stack is replayed as a movie sequence, occurred during acquisition of planes 6, 7, and 8. Overlaying the three planes as red, green, and blue (RGB) channels of an RGB image reveals the movement of one conoid (red arrow). After deconvolution, viewing the 3D reconstruction at an oblique angle reveals the distortion (red arrow; jagged profile of the conoid, which is actually round).