Images of Toxoplasma gondii expressing YFP-tubulin. (Top left) The raw wide-field (WF) image, (top right) the result obtained after deconvolution, (bottom left, bottom right) images obtained from different regions of the same sample using spinning-disk and spot-scanning confocal microscopes, respectively. The same specimen was imaged on all three microscopes using equivalent objective lenses and fluorescent filters. In all cases, the images shown are the sum of three adjacent focal planes spaced 0.3 µm apart. The cortical microtubules are visible as lines of intensity and the conoid as a bright dot at the apical end of each cell.