Quantitative comparison of 3D deconvolution with deblurring. A 3D stack of images of a DAPI-stained nucleus acquired on a wide-field microscope was processed by either full 3D constrained iterative deconvolution (CID) or deblurring using a nearest-neighbor algorithm (nnei). A single slice from the processed 3D stack, along with the corresponding unprocessed raw data, is shown. The graph shows the intensity profiles measured in a thin horizontal band across each image as indicated. Although both images have increased contrast relative to the raw data, note that the SNR is enhanced after 3D deconvolution but degraded with nearest-neighbor deblurring.