Protocol

Estimation of DNA Concentration in Forensic Samples Prior to Multiplex PCR Amplification

Adapted from Genetic Variation (ed. Weiner et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2007.

INTRODUCTION

This protocol describes the use of quantitative polymerase chain reaction (qPCR) for quantitation of DNA in forensic samples prior to PCR amplification. It is adapted from the Armed Forces DNA Identification Laboratory (Rockville, MD) Standard Operating Procedure.

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