Recipe

Tris buffer (1 M, pH 7.5)

Dissolve 6.05 g of Tris base in 30 mL of H2O. Adjust pH to 7.5 with 5 M HCl. Adjust final volume to 50 mL with H2O.

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  1. doi:10.1101/pdb.rec12394 Cold Spring Harb Protoc 2011: pdb.rec12394-
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