Recipe

Agar plates with LB medium and ampicillin (50 μg/mL)

LB (Luria-Bertani) liquid medium

Bacto agar

Ampicillin (100 mg/mL dissolved in H2O)

Dissolve 15 g of Bacto agar in 1.0 L of LB medium and sterilize by autoclaving. Cool to 50°C in a temperature-controlled water bath. Add 0.50 mL of 100 mg/mL ampicillin. Pour into plates.

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  1. doi:10.1101/pdb.rec12396 Cold Spring Harb Protoc 2011: pdb.rec12396-
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