Agar plates with LB medium and ampicillin (50 μg/mL)
LB (Luria-Bertani) liquid medium
Bacto agar
Ampicillin (100 mg/mL dissolved in H2O)
Dissolve 15 g of Bacto agar in 1.0 L of LB medium and sterilize by autoclaving. Cool to 50°C in a temperature-controlled water bath. Add 0.50 mL of 100 mg/mL ampicillin. Pour into plates.
