Carbonate-bicarbonate buffer stock (pH 9.2)

MATERIALS

METHOD

• 1. Prepare a 0.2-M solution of anhydrous sodium carbonate (2.2 g/100 mL).

• 2. Prepare a 0.2-M solution of sodium bicarbonate (1.68 g/100 mL).

• 3. Combine 4 mL of carbonate solution from Step 1 and 46 mL of bicarbonate solution from Step 2.

• 4. Bring to 200 mL with H₂O.

  Final pH will be 9.2.