Recipe

Embryonic extract dialysis buffer

20 mM Tris-Cl (pH 8.0)

50 mM KCl

1 mM dithiothreitol (DTT)

10% glycerol

0.5 mM EDTA

1 mM PMSF (phenylmethylsulfonyl fluoride)

The DTT and PMSF must be added fresh on the day of homogenization.

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  1. doi:10.1101/pdb.rec12400 Cold Spring Harb Protoc 2011: pdb.rec12400-
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