Recipe

Splicing buffer –ATP (5×)

175 mM KCl

20 mM MgCl2

7.5 mM dithiothreitol (DTT)

15% PEG 8000

Store at -70ºC to -80ºC.

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  1. doi:10.1101/pdb.rec12403 Cold Spring Harb Protoc 2011: pdb.rec12403-
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