Table 2. Adoptive transfer by injection route and cell type (mouse)
| Cell type |
Injection route(s) |
Injection volume |
Needle gauge |
Homing time |
Procedure recommendations |
|---|---|---|---|---|---|
| T-cell (naïve orcentralmemory) |
i.v.a (retro-orbital or tail vein) | 0.1-0.5 mL (adult animal) |
26-28 | LN/spleen: Early: 15 minEquilibrated: 6-8 h |
Gently restrain animal for tail vein injections; prewarm tail. Anesthetize animals for retro-orbital injections. |
| T-cell (effector/memory) |
i.p. |
0.25-1.5 mL | ≤20 | Practice firm, gentle grip on animal for i.p. injections (train with skilled professional before attempting). | |
| i.v. | 0.1-0.5 mL | 26-28 |
Early (i.v.): 1-3 h Equilibrated: 24 h |
||
| B cell | i.v. (retro-orbital or tail vein) | 0.1-0.5 mL |
26-28 | LN/spleen: Early: 2-4 hEquilibrated: 12 h |
Same as for T-cell injection |
| DCs | s.c. | 0.05-0.1 mL/site |
24-28 |
LN: Early: 18-24 hPeak: 36 h |
Anesthetize or restrain for subcutaneous injection. The number of DCs found in the LN is greatly improved by s.c. injections at sites where lymphatics drain to the LN of interest. |
| i.v. | 0.1-0.5 mL | 26-28 |
12-23 h (fewer cells home) |
||
| aAbbreviations: DC, dendritic cell; i.p., intraperitoneal; i.v., intravenous; LN, lymph node; s.c., subcutaneous. | |||||
