Preparation of Meiotic Chromosomes from Larval and Pupal Drosophila Testes
Adapted from Drosophila Protocols (ed. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
Several techniques have been developed for the preparation of Drosophila male meiotic chromosomes. All of these techniques allow a clear visualization of chromosomes, but not of other cellular structures such as the cytoskeleton and the spindle. This protocol describes two methods for preparation of meiotic chromosomes from larval and pupal testes. The first is a very simple method for preparing aceto-orcein-stained chromosomes. The second allows preparation of unstained chromosomes that can subsequently be stained with various dyes such as Giemsa, Hoechst 33258, and DAPI, or processed for in situ hybridization.
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