Plating Cells for Microinjection
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010.INTRODUCTION
The efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. This protocol describes the general procedures needed to culture cells for microinjection. Coverslips need to be marked so that microinjected cells can be identified at desired time points after injection. Coverslips can be etched by the user, as described here, or pre-etched coverslips can be purchased. Once the coverslips have been etched and sterilized, cells can be plated onto them and allowed to grow.
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