DNA Sample Preparation and Loading Sample into Pipettes for Microinjection of Cells
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010.INTRODUCTION
The efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. This protocol describes in general the preparation and loading of plasmid DNA for microinjections. Plasmid DNA can be purified by traditional means such as cesium chloride density ultracentrifugation, or by commercially available resin-based purification kits. The resulting preparation can then be delivered into microinjection needles either by backfilling or by a forward-filling approach.
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