Microinjecting Cells Using a Pulsed-Flow Microinjection System
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2010.INTRODUCTION
The efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. A pulsed-flow microinjection system, such as the Eppendorf InjectMan and FemtoJet systems, permits a high level of control over the microinjection parameters, ensuring reproducibility. The system is also easy to use, is rapid, and is less likely to damage cells than the constant-flow method.
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