Protocol

Megaselia abdita: Fixing and Devitellinizing Embryos

  1. Urs Schmidt-Ott2
  1. Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA

    INTRODUCTION

    This protocol describes a combined heat/formaldehyde fixation procedure, as well as a manual devitellinization procedure, for pregastrular Megaselia abdita embryos.

    Footnotes

    • ↵1 These authors contributed equally to this work.

    • ↵2 Corresponding author (uschmid{at}uchicago.edu).

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    1. doi:10.1101/pdb.prot5602 Cold Spring Harb Protoc 2011: pdb.prot5602- © 2011 Cold Spring Harbor Laboratory Press
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