Recipe

HL-3 for FlAsH-FALI

Reagent Final concentration (mM)
NaCl 110
KCl 5
NaHCO3 10
HEPES 5
Sucrose 30
Trehalose 5
MgCl2 10

  • Adjust the pH to 7.2 and the final volume to 1 L. Prepare fresh (from powder) the day of the experiment.

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    1. doi:10.1101/pdb.rec12427 Cold Spring Harb Protoc 2011: pdb.rec12427- © 2011 Cold Spring Harbor Laboratory Press
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