NP-40 Buffer for TAP

doi:10.1101/pdb.rec12432

Recipe

NP-40 Buffer for TAP

Reagent	Quantity (for 3 L)	Final concentration
Na₂HPO₄ (FW 142)	2.56 g	6 mM
NaH₂PO₄·H₂O (FW 138)	1.66 g	4 mM
NP-40 (100%)	30 mL	1%
NaCl	26.3 g	150 mM
EDTA (0.5 M)	12 mL	2 mM
NaF	6.3 g	50 mM
Leupeptin	12 mg	4 µg/μL
Na₃VO₄ (100 mM)	3 mL	0.1 mM

Bring to a final volume of 3 L with H₂O. Dispense into 500-mL aliquots. Just prior to use, add 10 COMPLETE EDTA-free protease inhibitor tablets (Roche), 1.3 mL of 0.5 M benzamidine, and 5.0 mL of 0.1 M PMSF to 500 mL of NP-40 buffer for TAP.